Ancillaries
Citrate Buffer For Heat-Induced Epitope Retrieval
Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues.
Overview
Formaldehyde fixation impairs or totally destroys the immunoreactivity of many antigens and epitopes. The negative effect of formaldehyde fixation can be reversed successfully with enzymatic digestion for some markers while not for others. Non-enzymatic epitope unmasking techniques have been recently introduced to improve the immunoreactivity of many antigens in formaldehyde fixed tissues. Heat-Induced Epitope Retrieval (HIER) in citrate buffer has been reported to improve the reactivity of many antibodies in formal-fixed tissues.
Products
| Product Number | Regulatory Status | Format | Volume | Action |
|---|---|---|---|---|
| AP-9003-125: Citrate Buffer | IVD | 10x stock solution; pH 6.0 | 125 ml | Request a Quote |
| AP-9003-500: Citrate Buffer | IVD | 10x stock solution; pH 6.0 | 500 ml | Request a Quote |
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